Improved estimation of urinary myoglobulin by counterimmunoelectrophoresis, as compared with the double immunodiffusion technique.
نویسندگان
چکیده
We describe a simple, sensitive, and relatively inexpensive counterimmunoelectrophoretic technique for detection and estimation of myoglobin. The test can be completed within 90 min, compared with 12-48 h required by double immunodiffusion. With the counterimmunoelectrophoretic technique we could detect myoglobin in concentrations of 2-3 mg/liter, while the detection limit by double immunodiffusion was about 9 mg/liter.
منابع مشابه
Detection of Aspergillus fumigatus serum precipitins by counterimmunoelectrophoresis.
Detection of serum precipitins to Aspergillus fumigatus by counterimmunoelectrophoresis is compared with the immunodiffusion technique. Eight of nine (89%) sera from patients with proven A. fumigatus infection were positive by both methods. No serum from subjects with other systemic mycoses, bacterial infections, or healthy controls had detectable precipitins. The highest serum precipitin titer...
متن کاملDifferentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique
Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. ...
متن کاملManual of signs, symptoms, methods and procedures for the assessment of the patient with SSc.
Anticentromere antibodies (ACA) Discrete speckled pattern on Hep-2 cells in interphase (8, 9). Anti-DNA topoisomerase I At a serum dilution of 1:40 by the Outcherlony double immunodiffusion method using as the antigen source a freshly prepared saline extract of lyophilized rabbit thymus acetone powder at a concentration of 20-40 mg protein/ml and a reference serum for comparison. Other procedur...
متن کاملAn Improved Motion Vector Estimation Approach for Video Error Concealment Based on the Video Scene Analysis
In order to enhance the accuracy of the motion vector (MV) estimation and also reduce the error propagation issue during the estimation, in this paper, a new adaptive error concealment (EC) approach is proposed based on the information extracted from the video scene. In this regard, the motion information of the video scene around the degraded MB is first analyzed to estimate the motion type of...
متن کاملComparison of histological and immunological techniques for detection of Pneumocystis carinii in rat bronchial lavage fluid.
We compared histological and immunological techniques in the early diagnosis of Pneumocystis carinii pneumonia in bronchial lavage fluid of steroid-treated rats. The rats were sacrificed weekly and lavage fluids were: (i) examined with cresyl echt violet and Giemsa stains; (ii) examined for P. carinii antigens by indirect fluorescent-antibody, counterimmunoelectrophoresis, and double-diffusion ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 21 6 شماره
صفحات -
تاریخ انتشار 1975